What are the steps in media preparation?
Procedure for preparation:
- Suspend 52.0g of powder in 1 litre of distilled or deionized water.
- Mix well.
- Heat to boiling for 1 minute to completely dissolve the powder.
- Cool to 45-500C and dispense into petri dishes.
- Pour 15-20 ml of the ready media on to petri dishes.
- Leave standing for thirty minutes to solidify.
How do you sterilize the media?
Sterilization of the media is most commonly achieved by applying heat and to a lesser extent by other means (physical methods, chemical treatment, and radiation). Heat sterilization: Heat is the most widely used sterilization technique.
Which is the best technique to sterilize the growth media?
autoclaving
Sterilization of Culture Media Bottled media is typically sterilized by autoclaving; whereas plate media is normally dispensed under ISO 14644 class 5 conditions and, for media used in cleanrooms, plates then sterilized by irradiation.
What is the purpose of media preparation?
To become familiar with the necessary nutritional and environmental factors for culturing microorganisms in the laboratory. To understand the decontamination or sterilization process using an autoclave. To learn the procedures used in preparing media needed for culturing microorganisms.
How are media sterilized before use?
Media preparation for the microbiology laboratory involves the use of an autoclave for sterilization, which permits exposure to high temperatures for a specified period of time. Generally, a temperature of 121°C (achieved by using steam at 15 lb/sq in) for 15 minutes is used to heat-sterilize bacteriological media.
What are sterilization techniques?
Sterilization can be achieved by a combination of heat, chemicals, irradiation, high pressure and filtration like steam under pressure, dry heat, ultraviolet radiation, gas vapor sterilants, chlorine dioxide gas etc.
Why do we need to sterilize media?
When microbiological media has been made, it still has to be sterilized because of microbial contamination from air, glassware, hands, etc. Within a few hours there will be thousands of bacteria reproducing in the media so it has to be sterilized quickly before the microbes start using the nutrients up.
What are types of sterilization?
The methods are: 1. Moist Heat Sterilization 2. Dry Heat Sterilization 3. Gas Sterilization and Others.