What is Baird-Parker agar used for?
Baird Parker Agar is used for the isolation and differentiation of coagulase-positive staphylococci in food and pharmaceuticals according to the Baird Parker method. As a nitrogen source for the organism, casein peptone and meat extract are added to the medium.
Which bacteria grow on Baird-Parker agar?
Baird-Parker agar is a type of agar used for the selective isolation of gram-positive Staphylococci species. It contains lithium chloride and tellurite to inhibit the growth of alternative microbial flora, while the included pyruvate and glycine promote the growth of Staphylococci.
How do you make Baird-Parker agar?
Heat with frequent agitation and boil for one minute to completely dissolve the medium. Autoclave at 121°C for 15 minutes. After cooling to 45- 50°C, add 50 mL of egg yolk tellurite supplement and 3 ml sterile 3.5% potassium tellurite solution or 50 ml egg yolk tellurite emulsion. Mix thoroughly before dispensing.
What are the selective media used for isolating Staphylococcus?
Two selective media (mannitol salt agar and Staphy- lococcus medium 110 agar) used for the isolation and enumeration of staphylococci depend on salt to inhibit non-staphylococci. Baird-Parker agar achieves its selectiv- ity through the addition of potassium tellurite and lithium chloride.
Is Baird Parker Agar selective?
Baird–parker agar is a selective medium for the enumeration of Staphylococcus aureus in foods and this was first reported by Baird–Parker. It is widely recommended by national and international bodies. Selectivity is attained with potassium tellurite and lithium chloride.
What Colour is Baird Parker Agar?
Canary yellow
Solution is light to medium amber, very slightly to slightly opalescent. Appearance: Canary yellow, opaque suspension with a resuspendable precipitate. Prepare the medium per label directions. Inoculate and incubate at 35 ± 2°C for 24-50 hours.
What makes Baird Parker Agar differential?
Baird Parker Agar was developed by Baird Parker from the Tellurite-glycine formulation of Zebovitz et al for isolation and enumeration of Staphylococci in food and other material since it allows a good differentiation of coagulase-positive strains.
What is Baird Parker Agar made of?
Baird Parker Agar contains casein peptone, beef extract and yeast extract as sources of nitrogen, carbon, sulfur, vitamins and trace elements. Glycine and lithium chloride : Have an inhibitory action against organisms other than S. aureus.
How would you distinguish between members of the genus Staphylococcus on Baird Parker?
Differentiation between members of the Genus Staphylococcus is possible: coagulase-positive staphylococci appear as shiny, gray-black, convex colonies with a clear zone surrounding the colonies; while coagulase- negative staphylococci grow poorly and appear as small, dull black colonies while clear or opaque zones are …
What is the best specimen for isolation of Staphylococcus?
To isolate staphylococci, clinical specimens are usually grown on Blood agar (described in Lab 14).
How is Baird Parker selective?
Publisher Summary. Baird–parker agar is a selective medium for the enumeration of Staphylococcus aureus in foods and this was first reported by Baird–Parker. It is widely recommended by national and international bodies. Selectivity is attained with potassium tellurite and lithium chloride.
How is Baird Parker Agar differential?
Baird-Parker Agar is a medium for the enumeration of Staphylococcus aureus in foods which was first reported by Baird-Parker (1962). It is a moderately selective differential medium used for the isolation and enumeration of Staphylococcus aureus from food, environmental and clinical samples.
How do you differentiate between Staphylococcus and Streptococcus?
Staphylococci and Streptococci are grouped as Gram-positive cocci. Staphylococci form clumps, whereas Streptococci grow in chains. They can be discriminated by catalase test because Staphylococci have the capability to produce catalase [2].
What is the confirmatory test for Staphylococcus aureus?
tube coagulase test
In many developing countries, the tube coagulase test is usually confirmatory for S. aureus and is routinely done using either human or sheep plasma. This study evaluated Mannitol salt agar and the deoxyribonuclease (DNase) test for improving the efficiency of the tube coagulase test in resource limited settings.
Which test would be best for differentiating between Streptococcus and Staphylococcus?
The catalase test is a particularly important test used to determine whether the Gram + cocci is a staphylococci or a streptococci.
What is the best test to differentiate between the genus Staphylococcus and Streptococcus?
The catalase test is important in distinguishing streptococci (catalase-negative) staphylococci which are catalase positive. The test is performed by flooding an agar slant or broth culture with several drops of 3% hydrogen peroxide. Catalase-positive cultures bubble at once.
How can you distinguish between Gram-positive Staphylococcus and Streptococcus?