Why is Microtechnique important?
Microtechnique is an important experimental science that has led and continues to lead a great service for each branch of the life sciences: microbiology, genetics, embryology, morphology and science, also plays an important role in the development of medical studies of human anatomy.
What is Microtechnique in biology?
(in biology), the aggregate of the methods and procedures used to study the structure, vital activity, development, chemical composition, and physical properties of cells, tissues, and organs by means of optical and electron microscopes.
Why is Microtechnique important in botany?
Methods used in plant micro-experiments. Botanical microtechnique is an aggregate of methods providing micro visualization of gene and gene product in an entire plant. Plant microtechnique is also a study providing valuable experimental information.
What are the different fixatives used in Microtechnique?
Feder and O’Brien (1968) reviewed principles and methods used in Plant Microtechnique, which introduced the concept of using non-coagulant fixatives such as osmium tetroxide, acrolein glutaral-dehyde and formaldehyde, coupled with the use of plastics such as glycol methacrylate polymer instead of wax.
What is Microtechnique in phlebotomy?
Nature of the Work The primary function of a Phlebotomy Technician is to obtain patient blood specimens by venipuncture or microtechniques. The Phlebotomy Technician aids in the collection and transportation of other laboratory specimens, and may be involved with patient data entry.
What is dehydration in Microtechnique?
Dehydration of tissues is the important process because of the paraffin, in which the tissues are embedded, is not miscible with water and does not penetrate the tissue effectively. Hence water in the tissue should be removed before embedding; this process is called as dehydration.
How is it prepared for use in the Celloidin embedding process?
Celloidin Embedding The tissue is dehydrated in alcohol in the same way as for paraffin except that it is transferred from absolute alcohol to a dilute solution of celloidin. As the alcohol and ether evaporate, they are replaced by more concentrated celloidin.
What is killing and fixing?
Ø Killing agent is the chemical reagent used for killing the plant specimens. Ø Fixation is the preservation of all structural and cellular elements in a biological specimen in as near their original state as possible. Ø Fixing agent is the chemical reagent used for fixing the plant specimens.
What is the best clearing agent?
Xylene
- The most commonly used clearing agent is xylene.
- Since 1950s, it is used as a safest alternative to dangerous chemicals such as aniline oil, benzene and chloroform.
What is clearing agent in histopathology?
CLEARING AGENTS are used throughout the histology lab in the processes of tissue and slide preparation—to remove alcohol and other dehydrants from tissues prior to embedding (usually in paraffin wax), and from finished slides prior to mounting.
What is Celloidin technique?
Celloidin is dissolved in equal parts of absolute alcohol and ether. The tissue is dehydrated in alcohol in the same way as for paraffin except that it is transferred from absolute alcohol to a dilute solution of celloidin. As the alcohol and ether evaporate, they are replaced by more concentrated celloidin.
What is Celloidin in histology?
Celloidin is a sulfated and nitrated cellulose which can be used in solution to infiltrate small to medium sized specimens. It can also be used in 0.5-1% solution as a covering over sections to retain difficult section on slides.
What is Post chroming?
Post chromation may also be referred to as post chroming or post chromatisation. It is the practice of treating tissues fixed in a primary fixative, usually one containing formalin or potassium dichromate, with a simple solution of potassium dichromate for an extended period, usually days and sometimes weeks.
What is the principle of fixation?
5.3 PRINCIPLE OF FIXATION Fixation results in denaturation and coagulation of protein in the tissues. The fixatives have a property of forming cross links between proteins, thereby forming a gel, keeping everything in their in vivo relation to each other.
What are the 2 groups of clearing agents?
Different types of clearing agents are chloroform, Xylene, Toluene, Paraffin, Methyl benzoate and methyl salicylate & Citrus fruit oils. The commonly used clearing agent is xylene that is miscible with both alcohol & parrafin wax.
Is xylene the best clearing agent?
1.) Xylene: It is the most commonly used clearing agent in histopathology laboratory.
Why is xylene used in histology?
Xylene has been generally used as a clearing and deparaffinizing agent in histology. Because of the potential toxic and flammable nature of xylene, its substitutes have been introduced into some laboratories.
What is Celloidin in histopathology?
What is microtechnique in biology?
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique.
What are the two types of microtechnique?
Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique. With respect to both plant microtechnique and animal microtechnique, four types of methods are commonly used, which are whole mounts, smears, squashes, and sections, in recent micro experiments.
What is zoological microtechnique?
The zoological microtechnique is the art of the preparation for microscopic animal observation. Although many microtechniques can be used in both plant and animal micro experiments. Some methods may differ from itself when employed in different field.
Which microtechnique is used in both plant and animal micro observation?
Some general microtechnique can be used in both plant and animal micro observation. Whole mounts, smears, squashes, and sections are four commonly used methods when preparing plant and animal specimens for specific purposes.